Twinkle ("Ambusher") (born September 12, 1998) is a 6 year old girl is one of Owli's enemies who are part of the Weegee Killers, she has a one-sided crush with Owli, but she tends to be enemies with him. Twinkle is Wayne's sister and Juani's sister in law, the close friend of Kip and the best friend of Eubie. Twinkle tends to like pink and her personality is calm, mischievious and sociable.

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